![]() Our web application hotSPOT provides a publicly available resource for researchers to design next-generation sequencing experiments to effectively study mutations in healthy tissues and cancer. Compared to the original genomic regions used for these studies, the regions identified by our algorithm improved mutation capture efficacy ranging from 9.6 to 12.1-fold. The efficacy of our algorithm was tested using three publicly available datasets. We have created an algorithm that formats mutation data into a targetable panel of genomic segments that can be used to design sequencing experiments. However, it is not economically feasible to sequence large genomic regions at the depth necessary to study mutations in healthy tissues. Next-generation sequencing has become the gold standard for studying cancer genomics. Many studies have shown that mutations in healthy tissue and cancer cluster at specific areas in the genome, often referred to as mutation hotspots. Mutations are present in healthy skin long before clinical signs of skin cancer arise.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |